Department of Microbiology

Services Offered

SAMPLES RECEIVED

Sputum, Stool, Blood, CSF, Catheter tips, All body fluids, Throat swabs, Pus, Urine, Corneal scrappings, Skin scraping, Hair, Nail.

Dr. D. Y. Patil Medical College, Hospital & Research Centre

MICROSCOPY

Bacterial – Gram Stain, Albert Stain, Wet mount, Darkground microscopy.
Mycobacterial – Ziehl-Neelsen
Fungal – Negative stain, KOH mount, Lactophenol cotton blue.
Parasitic – Saline mount, Iodine mount, Giemsa stain

CULTURE & SENSITIVITIES

Bacterial- Aerobic bacterial culture & sensitivity, Anaerobic bacterial culture
Mycobacterium culture & sensitivity
Fungal culture & sensitivity

MYCOLOGY

Generic identification & speciation of various fungal isolates by special phenotypic methods.
Vitek-2 for identification of yeasts & Antifungal sensitivity testing

PARASITOLOGY

Malaria antigen detection test
PBS for Malaria parasite, Microfilaria
Saline and Iodine mount for stool ova & Cyst

SURVEILLANCE

Aerobic & Anaerobic for Operation Theatres & Critical Care Units
MRSA surveillance of Medical & Paramedical staff.
Detection of ESBL, MBL, AmpC, Inducible Clindamycin among MDR strains

AIR SAMPLER

IMMUNOLOGY

R.A Factor Quantitative, ASO Quantitative, CRP Quantitative

VIROLOGY

HIV-1 & 2 test (Tridot,CMIA), HbsAg test ( ICT,CMIA ), Dengue IgM & IgG test, NS1 Ag ( ICT&ELISA ), HCV (ICT,CMIA ) , Chikungunya (ICT), Viral load of HIV assay by Real Time PCR
ICT-Immunochromatography, CMIA- Chemiluminescence Microparticle Immunoassay

VIDAS

ARCHITECT

SEROLOGICAL TESTS

WIDAL test, VDRL/RPR test, Brucella (SAT), leptospia IgG & IgM (ICT)

RAPID DIAGNOSIS OF BACTERIAL INFECTIONS

Bact/ Alert for faster detection of septicaemia, Enteric fever and bacterial endocarditis.
MB Bact for liquid culture of M. tuberculosis.
Rapid detection of causative agents and MIC of drugs by Vitek- 2 system.

RAPID DETECTION BY IMMUNOFLUORESCENCE MICROSCOPE

MOLECULAR BIOLOGY

Detection of M.tuberculosis and NTM by PCR & Hybridization as well as MDR-TB by molecular methods (LiPA). Result available within 6-8 hrs.
Gene- probe( rRNA amplified) method of diagnosis of M.tuberculosis by quantitative RNA method. Result available in 3 hrs.
Genexpert ( detects DNA) for M.tuberculosis with Rifampicin sensitivity. Results available within 3 hrs.

GeneXpert (CBNAAT)

PCR LABORATORY

NABL Accredited, ICMR approved, Fully Functional PCR Laboratory
rRT PCR COVID-19 testing

Biosafety Cabinets

Refrigerators

Deep Freezer (-70⁰C)

Automated Extractor

Thermocycler

CENTRAL RESEARCH LABORATORY

Confocal microscopy and Flow cytometry for Research purpose.

National programmes being carried out in the department

ICTC :- Prevention of Parent To Child Transmission of HIV (PPTCT) is a programme of MSACS with UNICEF. Dept of Microbiology carries out the serological part of the programme.One staff member is a qualified Trainer in this programme. We are also involved in training process of the health personnel, as this programme is now being extended up to primary health care centers. ICTC- Integrated Counselling and testing center.

RNTCP:-Revised National Tuberculosis Control Programme. The microscopy and sputum smear reporting is carried out in the Microbiology Dept.

Genexpert (CBNAAT, detects DNA) for M.tuberculosis with Rifampicin sensitivity. Results available within 3 hrs.