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Department of Microbiology

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Services Offered

MICROSCOPY

Bacterial – Gram’s Stain, Albert Stain, wet mount, Darkground microscopy.

Mycobacterial – Ziehl-Neelson

Fungal – Negative stain , KOH mount, Lactophenol cotton blue.

Parasitic – saline mount, Iodine mount, Giemsa stain

Dr. D. Y. Patil Medical College, Hospital & Research Centre
CULTURE & SENSITIVITIES

Aerobic bacterial culture & sensitivity, Anaerobic bacterial culture , Mycobacterium culture & sensitivity, Fungal culture & sensitivity.

Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre
SEROLOGICAL TESTS

HIV 1 & 2 test , WIDAL test, R.A Factor test, ELISA for Rotavirus, Hbs.Ag test, VDRL/RPR test, Brucella (SAT), leptospia IgG & IgM (ICT) ICT- Immunochromatographic test, ASO titre test, Dengue IgM & IgG test, Immunocomb test for HIV 1 & 2

Dr. D. Y. Patil Medical College, Hospital & Research Centre
SURVEILLANCE

Aerobic & Anaerobic for Operation Theatres & Critical Care Units. MRSA surveillance of Medical & Paramedical staff. Detection of ESBL, MBL, Inducible Clindamycin among MDR strains.

Dr. D. Y. Patil Medical College, Hospital & Research Centre

AIR SAMPLER

SAMPLES RECEIVED
Sputum, Stool, Blood, CSF, Catheter tips, All body fluids, Throat swabs, Pus, Urine, Corneal scrappings, Skin Clips, Hair , nail , Skin,
Dr. D. Y. Patil Medical College, Hospital & Research Centre
IMMUNOLOGY

R.A Factor Quantitative, ASO Quantitative, CRP Quantitative

PARASITOLOGY
Dr. D. Y. Patil Medical College, Hospital & Research Centre
Malaria antigen detection test, PBS for MP, Microfilaria, Saline and Iodine prep for stool ova & Cyst
VIROLOGY

HIV-1 & 2 test (Tridot,CMIA), HbsAg test ( ICT,CMIA ), Dengue IgM & IgG test, NS1 Ag ( ICT&ELISA ), HCV (ICT,CMIA ) , Chikungunya (ICT), Viral load of HIV assay by Real Time PCR

CMIA- Chemiluminescence Microparticle Immunoassay

Dr. D. Y. Patil Medical College, Hospital & Research Centre

VIDAS

Dr. D. Y. Patil Medical College, Hospital & Research Centre

ARCHITECT

MYCOLOGY

Generic identification & speciation of various fungal isolates by special phenotypic methods. Vitek-2 for identification of yeasts. & Antifungal sensitivity testing

Dr. D. Y. Patil Medical College, Hospital & Research Centre
MOLECULAR BIOLOGY

Detection of M.tuberculosis and NTM by PCR & Hybridization as well as MDR-TB by molecular methods (LiPA). Result available within 6-8 hrs. Gene- probe( rRNA amplified) method of diagnosis of M.tuberculosis by quantitative RNA method. Result available in 3 hrs.

Genexpert ( detects DNA) for M.tuberculosis with Rifampicin sensitivity. Results available within 3 hrs.

Dr. D. Y. Patil Medical College, Hospital & Research Centre

GeneXpert (CBNAAT)

RAPID DIAGNOSIS OF BACTERIAL INFECTIONS

Bact/ Alert is procured for faster detection of septicaemia, Enteric fever and bacterial endocarditis. MB Bact for liquid culture of M. tuberculosis. Rapid detection of causative agents and MIC of drugs by Vitek- 2 system.

Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre
RAPID DETECTION BY IMMUNOFLUORESCENCE MICROSCOPE
Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre
CENTRAL RESEARCH LABORATORY

For Use of Confocal microscopy and Flow cytometry for Research purpose.

National programmes being carried out in the department

ICTC :- Prevention of Parent To Child Transmission of HIV (PPTCT) is a programme of MSACS with UNICEF. Dept of Microbiology carries out the serological part of the programme.One staff member is a qualified Trainer in this programme. We are also involved in training process of the health personnel, as this programme is now being extended up to primary health care centers. ICTC- Integrated Counselling and testing center.

RNTCP:- Revised National Tuberculosis Control Programme The microscopy and sputum smear reporting is carried out in the Microbiology Dept.