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Department of Microbiology

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Services Offered

SAMPLES RECEIVED
Sputum, Stool, Blood, CSF, Catheter tips, All body fluids, Throat swabs, Pus, Urine, Corneal scrappings, Skin scraping, Hair, Nail.
Dr. D. Y. Patil Medical College, Hospital & Research Centre
MICROSCOPY
  • Bacterial – Gram Stain, Albert Stain, Wet mount, Darkground microscopy.
  • Mycobacterial – Ziehl-Neelsen
  • Fungal – Negative stain, KOH mount, Lactophenol cotton blue.
  • Parasitic – Saline mount, Iodine mount, Giemsa stain
Dr. D. Y. Patil Medical College, Hospital & Research Centre
CULTURE & SENSITIVITIES
  • Bacterial- Aerobic bacterial culture & sensitivity, Anaerobic bacterial culture
  • Mycobacterium culture & sensitivity
  • Fungal culture & sensitivity
Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre
MYCOLOGY
  • Generic identification & speciation of various fungal isolates by special phenotypic methods.
  • Vitek-2 for identification of yeasts & Antifungal sensitivity testing
Dr. D. Y. Patil Medical College, Hospital & Research Centre
PARASITOLOGY
Dr. D. Y. Patil Medical College, Hospital & Research Centre
  • Malaria antigen detection test
  • PBS for Malaria parasite, Microfilaria
  • Saline and Iodine mount for stool ova & Cyst
SURVEILLANCE
  • Aerobic & Anaerobic for Operation Theatres & Critical Care Units
  • MRSA surveillance of Medical & Paramedical staff.
  • Detection of ESBL, MBL, AmpC, Inducible Clindamycin among MDR strains
Dr. D. Y. Patil Medical College, Hospital & Research Centre

AIR SAMPLER

IMMUNOLOGY
  • R.A Factor Quantitative, ASO Quantitative, CRP Quantitative
VIROLOGY
  • HIV-1 & 2 test (Tridot,CMIA), HbsAg test ( ICT,CMIA ), Dengue IgM & IgG test, NS1 Ag ( ICT&ELISA ), HCV (ICT,CMIA ) , Chikungunya (ICT), Viral load of HIV assay by Real Time PCR
    ICT-Immunochromatography, CMIA- Chemiluminescence Microparticle Immunoassay
Dr. D. Y. Patil Medical College, Hospital & Research Centre

VIDAS

Dr. D. Y. Patil Medical College, Hospital & Research Centre

ARCHITECT

SEROLOGICAL TESTS
  • WIDAL test, VDRL/RPR test, Brucella (SAT), leptospia IgG & IgM (ICT)
Dr. D. Y. Patil Medical College, Hospital & Research Centre
RAPID DIAGNOSIS OF BACTERIAL INFECTIONS
  • Bact/ Alert for faster detection of septicaemia, Enteric fever and bacterial endocarditis.
  • MB Bact for liquid culture of M. tuberculosis.
  • Rapid detection of causative agents and MIC of drugs by Vitek- 2 system.
Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre
RAPID DETECTION BY IMMUNOFLUORESCENCE MICROSCOPE
Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre
MOLECULAR BIOLOGY
  • Detection of M.tuberculosis and NTM by PCR & Hybridization as well as MDR-TB by molecular methods (LiPA). Result available within 6-8 hrs.
  • Gene- probe( rRNA amplified) method of diagnosis of M.tuberculosis by quantitative RNA method. Result available in 3 hrs.
  • Genexpert ( detects DNA) for M.tuberculosis with Rifampicin sensitivity. Results available within 3 hrs.
Dr. D. Y. Patil Medical College, Hospital & Research Centre

GeneXpert (CBNAAT)

PCR LABORATORY
  • NABL Accredited, ICMR approved, Fully Functional PCR Laboratory
  • rRT PCR COVID-19 testing
Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre

Biosafety Cabinets

Dr. D. Y. Patil Medical College, Hospital & Research Centre

Refrigerators

Dr. D. Y. Patil Medical College, Hospital & Research Centre

Refrigerators

Dr. D. Y. Patil Medical College, Hospital & Research Centre

Deep Freezer (-70⁰C)

Dr. D. Y. Patil Medical College, Hospital & Research Centre
Dr. D. Y. Patil Medical College, Hospital & Research Centre

Automated Extractor

Dr. D. Y. Patil Medical College, Hospital & Research Centre

Thermocycler

CENTRAL RESEARCH LABORATORY
  • Confocal microscopy and Flow cytometry for Research purpose.
National programmes being carried out in the department

ICTC :- Prevention of Parent To Child Transmission of HIV (PPTCT) is a programme of MSACS with UNICEF. Dept of Microbiology carries out the serological part of the programme.One staff member is a qualified Trainer in this programme. We are also involved in training process of the health personnel, as this programme is now being extended up to primary health care centers. ICTC- Integrated Counselling and testing center.

RNTCP:-Revised National Tuberculosis Control Programme. The microscopy and sputum smear reporting is carried out in the Microbiology Dept.

Genexpert (CBNAAT, detects DNA) for M.tuberculosis with Rifampicin sensitivity. Results available within 3 hrs.

NABL Accredited Service Laboratory